畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (10): 1616-1621.doi: 10.11843/j.issn.0366-6964.2014.10.006

• 遗传繁育 • 上一篇    下一篇

汶上芦花鸡种质资源的遗传多样性分析

杜胭脂1,2,陈玉霞1,康丽1,徐来祥2,唐辉1,姜运良1*   

  1. (1.山东农业大学动物科技学院/动物医学院 动物分子遗传实验室,泰安 271018;2.曲阜师范大学生命科学学院,曲阜 273165)
  • 收稿日期:2014-01-25 出版日期:2014-10-23 发布日期:2014-10-23
  • 通讯作者: 姜运良,E-mail: yljiang723@aliyun.com
  • 作者简介:杜胭脂(1989-),女,山东临沂人,硕士生,主要从事动物分子遗传学研究,Tel:0538-8241593, E-mail:lyusk08@126.com
  • 基金资助:

    山东省农业良种工程(畜禽种质资源收集、保护与评价)

Analysis on the Genetic Diversity of Wenshang Barred Chicken Breed

DU Yan-zhi1,2,CHEN Yu-xia1,KANG Li1,XU Lai-xiang2,TANG Hui1,JIANG Yun-liang1*   

  1. (1.Laboratory of Animal Molecular Genetics,College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai’an 271018,China;2.College of Life Sciences,Qufu Normal University,Qufu 273165,China)
  • Received:2014-01-25 Online:2014-10-23 Published:2014-10-23

摘要:

为了评估汶上芦花鸡群体的遗传多样性,本研究采用联合国粮农组织(FAO)和国际动物遗传学会(ISAG)联合推荐的27对微卫星引物,对济宁汶上芦花鸡的100个个体(60只母鸡,40只公鸡)进行了遗传多样性检测,分析了该群体在DNA水平上的遗传多样性。结果,共检测到86个等位基因,每个微卫星座位的等位基因数从2个到6个不等,平均等位基因数为3.185,平均期望杂合度和多态信息含量分别为0.465 5和0.402 3。对分布在不同地方的3个群体的遗传距离分析表明,群体间的遗传距离为0.05~0.10。以上结果表明,汶上芦花鸡群体的遗传多样性偏低,必须采取一定的措施保护这一优良地方品种。

Abstract:

In this study,one hundred chickens including 60 hens and 40 roosters were sampled for the evaluation of genetic diversity on Wenshang Barred chicken breed by using 27 pairs of primers recommended jointly by FAO (Food and Agriculture) and ISAG (the International Society for Animal Genetic).The genetic diversity at DNA level were analyzed in this study.Totally 86 alleles were detected,the number of alleles varied from 2 to 6,giving a mean value of 3.185 alleles per locus.The average heterozygosity and polymorphism information content of 27 microsatellite loci were 0.465 5 and 0.402 3,respectively.The genetic distances between 3 populations distributed in different areas vary from 0.05 to 0.10.The results indicated that Wenshang Barred chicken breed had low heterozygosity and polymorphism information content,which imply that effective methods should be taken to protect this breed.

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